Loading…
Title

Chromatography - bio-rad laboratories

Description
Different types of chromatography such as ion exchange, gel filtration, reverse phase, and affinity have been used for the separation of plasmid dna.
/2
Full Text
Cheryl S Aberin and Samuel G Franklin, PhD,Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USAIntroductionPlasmid DNA is being used successfully as a gene deliveryvector in a variety of clinical applications (Smith et al. 1999).Plasmids for gene therapy are usually produced in an E. colihost. One of the technical challenges associated withproducing plasmid DNA of gene therapy grade is theremoval of contaminants such as bacterial chromosomalDNA, RNA, host proteins, and endotoxin.Chromatography plays a key role in the large-scalepurification of plasmids, both as a process step and asan analytical tool. Different types of chromatography suchas ion exchange, gel filtration, reverse phase, and affinityhave been used for the separation of plasmid DNA.Chromatography is often preceded by RNase treatment,diafiltration, precipitation, dilution, and other steps, whichcan increase process costs, decrease productivity andproduct recovery. Thus, there is a significant need for anal...