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Biochemical and ultrastructural properties of a mitochondrial ...

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By tl chan · 1970 · cited by 148 — treatment of the inner membrane matrix fraction of rat liver mitochondria with the ... the lubrol-insoluble fraction was enriched in.
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BIOCHEMICAL AND ULTRASTRUCTURALPROPERTIES OF A MITOCHONDRIAL INNERMEMBRANE FRACTION DEFICIENT IN OUTERMEMBRANE AND MATRIX ACTIVITIEST . L . CHAN, JOHN W . GREENAWALT, and PETER L . PEDERSENFrom the Department of Physiological Chemistry, The Johns Hopkins School of Medicine, Baltimore,Maryland 921205 . Dr . Chan's present address is the Department of Medicine, The New YorkUniversity Medical Center, New York 10016ABSTRACTTreatment of the inner membrane matrix fraction of rat liver mitochondria with thenonionic detergent Lubrol WX solubilized about 70% of the total protein and 90% ormore of the following matrix activities : malate dehydrogenase, glutamate dehydrogenase,and isocitrate dehydrogenase (NADP) . The Lubrol-insoluble fraction was enriched incytochromes, phospholipids, and a Mg++-stimulated ATPase activity . Less than 2% ofthe total mitochondrial activity of monoamine oxidase, an outer membrane marker, oradenylate kinase, an intracristal space marker could be detected in this inn...
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