9-lipids - saponification.pdf - mrs. iufer

Laboratory by a process called saponification – where the hydrolysis is carried out in the presence of a strong base (such as naoh or koh). 12 pages
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REV. 03/05 1CHM 112 Lab Procedure 9:LIPIDS: SAPONIFICATION(THE PROPERTIES AND SYNTHESIS OF SOAP)Objectives:(a) to examine the interaction of water and oil in the presence and absence of soap anddetergent(b) to examine the interaction of soap and de tergent solutions with different metal ionsolutions(c) to prepare a soap from common lipid sources(d) to determine the alkalinity of a commercially-prepared soapLipids are one of the four major classes of biomolecules (along with carbohydrates,proteins and nucleic ands). They store energy in the body for use when food is notreadily available. They are integral for cell membranes to separate compartmentswithin the cell, and can act as chemical messengers in the endocrine system andthroughout the body.Lipids are naturally occurring organic molecules that can dissolve in non-polar solvents,liker ether. The other biomolecules (proteins, carbohydrates and nucleic acids) arepolar and therefore much more soluble in a polar solvent like water.Many l...